Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976430..2977078 Replicon chromosome
Accession NZ_CP023606
Organism Mycobacterium tuberculosis strain LE103

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP462_RS14530 Protein ID WP_003899414.1
Coordinates 2976430..2976753 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP462_RS14535 Protein ID WP_003899415.1
Coordinates 2976833..2977078 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP462_RS14510 2972004..2973265 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP462_RS14515 2973639..2975078 - 1440 WP_003899411.1 phage major capsid protein -
CP462_RS14520 2975086..2975619 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP462_RS14525 2975772..2976263 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP462_RS14530 2976430..2976753 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP462_RS14535 2976833..2977078 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP462_RS14540 2977075..2978502 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP462_RS14545 2978504..2978896 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP462_RS14550 2978893..2979153 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP462_RS14555 2979170..2979532 - 363 WP_003900543.1 hypothetical protein -
CP462_RS14560 2979535..2980662 - 1128 WP_003899420.1 site-specific integrase -
CP462_RS14565 2980807..2981034 - 228 WP_003899421.1 hypothetical protein -
CP462_RS14570 2981031..2981420 - 390 WP_003899422.1 hypothetical protein -
CP462_RS14575 2981326..2981598 + 273 WP_003900544.1 hypothetical protein -
CP462_RS14580 2981697..2981930 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970395..2980662 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T83716 WP_003899414.1 NZ_CP023606:c2976753-2976430 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T83716 NZ_CP023606:c2976753-2976430 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT83716 WP_003899415.1 NZ_CP023606:c2977078-2976833 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT83716 NZ_CP023606:c2977078-2976833 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References