Detailed information of TA system TA082794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2266716..2267635
• Replicon: chromosome
• Accession: NZ_CP023591
• Organism: Mycobacterium tuberculosis strain TBV5365

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: CP447_RS11015
• Protein ID: WP_096872173.1
• Coordinates: 2267030..2267635 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: CP447_RS11010
• Protein ID: WP_003410124.1
• Coordinates: 2266716..2267021 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP447_RS10975	2261727..2262983	-	1257	WP_003410095.1	HNH endonuclease	-
CP447_RS10985	2263337..2263912	+	576	WP_096872171.1	hypothetical protein	-
CP447_RS10990	2263909..2264949	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
CP447_RS10995	2265191..2265910	+	720	WP_003410108.1	DUF433 domain-containing protein	-
CP447_RS11000	2265900..2266316	+	417	WP_003410114.1	hypothetical protein	-
CP447_RS11005	2266332..2266631	-	300	WP_096863739.1	hypothetical protein	-
CP447_RS11010	2266716..2267021	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
CP447_RS11015	2267030..2267635	-	606	WP_096872173.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP447_RS11020	2267660..2268019	-	360	WP_003410131.1	hypothetical protein	-
CP447_RS11025	2268179..2268637	-	459	Protein_2089	hypothetical protein	-
CP447_RS11030	2268634..2270151	-	1518	Protein_2090	DEAD/DEAH box helicase	-
CP447_RS11035	2270661..2271659	-	999	WP_003410146.1	cation diffusion facilitator family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T82794 WP_096872173.1 NZ_CP023591:c2267635-2267030 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T82794 NZ_CP023591:c2267635-2267030 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT82794 WP_003410124.1 NZ_CP023591:c2267021-2266716 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT82794 NZ_CP023591:c2267021-2266716 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV30