Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976378..2977026 Replicon chromosome
Accession NZ_CP023589
Organism Mycobacterium tuberculosis strain TBV5000

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP445_RS14535 Protein ID WP_003899414.1
Coordinates 2976378..2976701 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP445_RS14540 Protein ID WP_003899415.1
Coordinates 2976781..2977026 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP445_RS14515 2971952..2973213 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP445_RS14520 2973587..2975026 - 1440 WP_003899411.1 phage major capsid protein -
CP445_RS14525 2975034..2975567 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP445_RS14530 2975720..2976211 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP445_RS14535 2976378..2976701 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP445_RS14540 2976781..2977026 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP445_RS14545 2977023..2978450 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP445_RS14550 2978452..2978844 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP445_RS14555 2978841..2979101 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP445_RS14560 2979118..2979480 - 363 WP_003900543.1 hypothetical protein -
CP445_RS14565 2979483..2980610 - 1128 WP_003899420.1 site-specific integrase -
CP445_RS14570 2980755..2980982 - 228 WP_003899421.1 hypothetical protein -
CP445_RS14575 2980979..2981368 - 390 WP_003899422.1 hypothetical protein -
CP445_RS14580 2981274..2981546 + 273 WP_003900544.1 hypothetical protein -
CP445_RS14585 2981645..2981878 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970343..2980610 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T82682 WP_003899414.1 NZ_CP023589:c2976701-2976378 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T82682 NZ_CP023589:c2976701-2976378 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT82682 WP_003899415.1 NZ_CP023589:c2977026-2976781 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT82682 NZ_CP023589:c2977026-2976781 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References