Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976251..2976899 Replicon chromosome
Accession NZ_CP023588
Organism Mycobacterium tuberculosis strain TBDM425

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP444_RS14540 Protein ID WP_003899414.1
Coordinates 2976251..2976574 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP444_RS14545 Protein ID WP_003899415.1
Coordinates 2976654..2976899 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP444_RS14520 2971825..2973086 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP444_RS14525 2973460..2974899 - 1440 WP_003899411.1 phage major capsid protein -
CP444_RS14530 2974907..2975440 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP444_RS14535 2975593..2976084 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP444_RS14540 2976251..2976574 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP444_RS14545 2976654..2976899 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP444_RS14550 2976896..2978323 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP444_RS14555 2978325..2978717 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP444_RS14560 2978714..2978974 - 261 WP_014585547.1 helix-turn-helix domain-containing protein -
CP444_RS14565 2978991..2979353 - 363 WP_003900543.1 hypothetical protein -
CP444_RS14570 2979356..2980483 - 1128 WP_003899420.1 site-specific integrase -
CP444_RS14575 2980628..2980855 - 228 WP_003899421.1 hypothetical protein -
CP444_RS14580 2980852..2981241 - 390 WP_003899422.1 hypothetical protein -
CP444_RS14585 2981147..2981419 + 273 WP_003900544.1 hypothetical protein -
CP444_RS14590 2981518..2981751 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970216..2980483 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T82621 WP_003899414.1 NZ_CP023588:c2976574-2976251 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T82621 NZ_CP023588:c2976574-2976251 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT82621 WP_003899415.1 NZ_CP023588:c2976899-2976654 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT82621 NZ_CP023588:c2976899-2976654 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References