Detailed information of TA system TA082369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2266628..2267547
• Replicon: chromosome
• Accession: NZ_CP023584
• Organism: Mycobacterium tuberculosis strain MDRDM627

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: CP440_RS11030
• Protein ID: WP_003906719.1
• Coordinates: 2266942..2267547 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: CP440_RS11025
• Protein ID: WP_096865498.1
• Coordinates: 2266628..2266933 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP440_RS10990	2261639..2262895	-	1257	WP_003410095.1	HNH endonuclease	-
CP440_RS11000	2263249..2263824	+	576	WP_003410100.1	hypothetical protein	-
CP440_RS11005	2263821..2264861	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
CP440_RS11010	2265103..2265822	+	720	WP_003410108.1	DUF433 domain-containing protein	-
CP440_RS11015	2265812..2266228	+	417	WP_096865497.1	hypothetical protein	-
CP440_RS11020	2266244..2266543	-	300	WP_096863739.1	hypothetical protein	-
CP440_RS11025	2266628..2266933	-	306	WP_096865498.1	XRE family transcriptional regulator	Antitoxin
CP440_RS11030	2266942..2267547	-	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP440_RS11035	2267572..2267931	-	360	WP_003410131.1	hypothetical protein	-
CP440_RS11040	2268091..2268549	-	459	Protein_2092	hypothetical protein	-
CP440_RS11045	2268546..2270063	-	1518	Protein_2093	DEAD/DEAH box helicase	-
CP440_RS11050	2270573..2271571	-	999	WP_096865499.1	cation diffusion facilitator family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-91)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T82369 WP_003906719.1 NZ_CP023584:c2267547-2266942 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T82369 NZ_CP023584:c2267547-2266942 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGCGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11163.78 Da
• Isoelectric Point: 10.7482

>AT82369 WP_096865498.1 NZ_CP023584:c2266933-2266628 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAVNTLRKYVS
ALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT82369 NZ_CP023584:c2266933-2266628 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCATAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A654U351