82318

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976402..2977050 Replicon chromosome
Accession NZ_CP023583
Organism Mycobacterium tuberculosis strain MDRDM260

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP439_RS14540 Protein ID WP_003899414.1
Coordinates 2976402..2976725 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP439_RS14545 Protein ID WP_003899415.1
Coordinates 2976805..2977050 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP439_RS14520 2971976..2973237 + 1262 WP_096868035.1 IS3-like element IS987 family transposase -
CP439_RS14525 2973611..2975050 - 1440 WP_003899411.1 phage major capsid protein -
CP439_RS14530 2975058..2975591 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP439_RS14535 2975744..2976235 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP439_RS14540 2976402..2976725 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP439_RS14545 2976805..2977050 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP439_RS14550 2977047..2978474 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP439_RS14555 2978476..2978868 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP439_RS14560 2978865..2979125 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP439_RS14565 2979142..2979504 - 363 WP_003900543.1 hypothetical protein -
CP439_RS14570 2979507..2980634 - 1128 WP_003899420.1 site-specific integrase -
CP439_RS14575 2980779..2981006 - 228 WP_003899421.1 hypothetical protein -
CP439_RS14580 2981003..2981392 - 390 WP_003899422.1 hypothetical protein -
CP439_RS14585 2981298..2981570 + 273 WP_003900544.1 hypothetical protein -
CP439_RS14590 2981669..2981902 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970367..2980634 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(14-278)

Antitoxin

Transcriptional regulator AbiEi antitoxin, N-terminal domain

(1-93)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T82318 WP_003899414.1 NZ_CP023583:c2976725-2976402 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T82318 NZ_CP023583:c2976725-2976402 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT82318 WP_003899415.1 NZ_CP023583:c2977050-2976805 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT82318 NZ_CP023583:c2977050-2976805 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References