Detailed information of TA system TA081658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3637258..3637933
• Replicon: chromosome
• Accession: NZ_CP023569
• Organism: Enterobacter hormaechei strain BW

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CPT31_RS17130
• Protein ID: WP_062937994.1
• Coordinates: 3637258..3637557 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: CPT31_RS17135
• Protein ID: WP_015571639.1
• Coordinates: 3637568..3637933 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT31_RS17120	3633635..3635827	+	2193	WP_017382756.1	type I secretion system permease/ATPase	-
CPT31_RS17125	3635847..3637007	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
CPT31_RS17130	3637258..3637557	+	300	WP_062937994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPT31_RS17135	3637568..3637933	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
CPT31_RS17140	3637960..3639141	-	1182	WP_017382758.1	PLP-dependent aminotransferase family protein	-
CPT31_RS17145	3639162..3640049	-	888	WP_015571641.1	LysR family transcriptional regulator	-
CPT31_RS17150	3640147..3640749	+	603	WP_017382759.1	short chain dehydrogenase	-
CPT31_RS17155	3640746..3641477	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11658.30 Da
• Isoelectric Point: 9.6776

>T81658 WP_062937994.1 NZ_CP023569:3637258-3637557 [Enterobacter hormaechei]
MINHFRDQWFEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T81658 NZ_CP023569:3637258-3637557 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGTTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT81658 WP_015571639.1 NZ_CP023569:3637568-3637933 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT81658 NZ_CP023569:3637568-3637933 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCATTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9