Detailed information of TA system TA081605

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3673..3943
• Replicon: plasmid pFORC43
• Accession: NZ_CP023552
• Organism: Escherichia coli strain FORC_043

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FORC43_RS27580
• Protein ID: WP_001388592.1
• Coordinates: 3785..3943 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	3673..3736 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC43_RS27555	137..1804	-	1668	WP_001091724.1	group II intron reverse transcriptase/maturase	-
FORC43_RS27570	2903..3505	+	603	Protein_1	plasmid SOS inhibition protein A	-
-	3517..3741	+	225	NuclAT_0	-	-
-	3517..3741	+	225	NuclAT_0	-	-
-	3517..3741	+	225	NuclAT_0	-	-
-	3517..3741	+	225	NuclAT_0	-	-
FORC43_RS27575	3526..3705	-	180	WP_001309233.1	hypothetical protein	-
-	3673..3736	-	64	-	-	Antitoxin
FORC43_RS27580	3785..3943	+	159	WP_001388592.1	type I toxin-antitoxin system Hok family toxin	Toxin
FORC43_RS28575	4181..4746	-	566	Protein_4	hypothetical protein	-
FORC43_RS27595	4925..5746	+	822	WP_001234482.1	DUF945 domain-containing protein	-
FORC43_RS27605	6041..6631	-	591	WP_176453891.1	transglycosylase SLT domain-containing protein	-
FORC43_RS27610	6971..7354	+	384	WP_001151566.1	relaxosome protein TraM	-
FORC43_RS27615	7548..8240	+	693	WP_000332477.1	PAS domain-containing protein	-
FORC43_RS27620	8328..8555	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	agg3D / agg3C / agg3C / agg3C / agg3B / agg3A / aap/aspU / pic	1..77422	77422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6135.36 Da
• Isoelectric Point: 10.1101

>T81605 WP_001388592.1 NZ_CP023552:3785-3943 [Escherichia coli]
MKLPRSSLVWRVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T81605 NZ_CP023552:3785-3943 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGCGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT81605 NZ_CP023552:c3736-3673 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Antitoxin