Detailed information of TA system TA081603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4314493..4315292
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: CR208_RS21045
• Protein ID: WP_021293021.1
• Coordinates: 4314493..4314957 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CR208_RS21050
• Protein ID: WP_001307405.1
• Coordinates: 4314957..4315292 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS21015	4309494..4309928	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
CR208_RS21020	4309946..4310824	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CR208_RS21025	4310814..4311593	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CR208_RS21030	4311604..4312077	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CR208_RS21035	4312100..4313380	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CR208_RS21040	4313629..4314438	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CR208_RS21045	4314493..4314957	-	465	WP_021293021.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CR208_RS21050	4314957..4315292	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CR208_RS21055	4315441..4317012	-	1572	WP_001273741.1	galactarate dehydratase	-
CR208_RS21060	4317387..4318721	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CR208_RS21065	4318737..4319507	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T81603 WP_021293021.1 NZ_CP023541:c4314957-4314493 [Escherichia coli O104:H21 str. CFSAN002236]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Nucleotide

• Length: 465 bp

>T81603 NZ_CP023541:c4314957-4314493 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCTTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT81603 WP_001307405.1 NZ_CP023541:c4315292-4314957 [Escherichia coli O104:H21 str. CFSAN002236]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT81603 NZ_CP023541:c4315292-4314957 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7