Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2925136..2925638 Replicon chromosome
Accession NZ_CP023541
Organism Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CR208_RS14235 Protein ID WP_000767829.1
Coordinates 2925136..2925390 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CR208_RS14240 Protein ID WP_001259255.1
Coordinates 2925387..2925638 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR208_RS14205 2920151..2920378 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CR208_RS14210 2920392..2921450 - 1059 WP_000492340.1 transport protein YeeE -
CR208_RS14215 2921629..2922987 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CR208_RS14220 2922977..2923039 - 63 WP_010723108.1 membrane protein YoeI -
CR208_RS14225 2923254..2924183 - 930 WP_000803351.1 LysR family transcriptional regulator -
CR208_RS14230 2924229..2925053 - 825 WP_000754749.1 SDR family oxidoreductase -
CR208_RS14235 2925136..2925390 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CR208_RS14240 2925387..2925638 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CR208_RS14245 2925921..2925971 + 51 WP_001364200.1 his operon leader peptide -
CR208_RS14250 2926117..2927016 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CR208_RS14255 2927022..2928326 + 1305 WP_021293346.1 histidinol dehydrogenase -
CR208_RS14260 2928323..2929393 + 1071 WP_000108973.1 histidinol-phosphate transaminase -
CR208_RS14265 2929393..2930460 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T81598 WP_000767829.1 NZ_CP023541:c2925390-2925136 [Escherichia coli O104:H21 str. CFSAN002236]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T81598 NZ_CP023541:c2925390-2925136 [Escherichia coli O104:H21 str. CFSAN002236]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT81598 WP_001259255.1 NZ_CP023541:c2925638-2925387 [Escherichia coli O104:H21 str. CFSAN002236]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT81598 NZ_CP023541:c2925638-2925387 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References