81585

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1038611..1039152 Replicon chromosome
Accession NZ_CP023541
Organism Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CR208_RS04935 Protein ID WP_000615976.1
Coordinates 1038611..1038889 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CR208_RS04940 Protein ID WP_000729704.1
Coordinates 1038892..1039152 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR208_RS04920 1036193..1036771 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CR208_RS04925 1036977..1037744 + 768 WP_000333380.1 class II glutamine amidotransferase -
CR208_RS04930 1037715..1038455 - 741 WP_001225679.1 murein L,D-transpeptidase -
CR208_RS04935 1038611..1038889 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CR208_RS04940 1038892..1039152 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CR208_RS04945 1039338..1040111 + 774 WP_000543899.1 C40 family peptidase -
CR208_RS04950 1040168..1040524 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CR208_RS04955 1040517..1040795 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CR208_RS04960 1040900..1042612 - 1713 Protein_936 flagellar biosynthesis protein FlhA -
CR208_RS04965 1042584..1043369 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T81585 WP_000615976.1 NZ_CP023541:c1038889-1038611 [Escherichia coli O104:H21 str. CFSAN002236]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T81585 NZ_CP023541:c1038889-1038611 [Escherichia coli O104:H21 str. CFSAN002236]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT81585 WP_000729704.1 NZ_CP023541:c1039152-1038892 [Escherichia coli O104:H21 str. CFSAN002236]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT81585 NZ_CP023541:c1039152-1038892 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References