Detailed information of TA system TA081582

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 483462..483959
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: CR208_RS02235
• Protein ID: WP_001173343.1
• Coordinates: 483786..483959 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: CR208_RS02230
• Protein ID: WP_000398619.1
• Coordinates: 483462..483758 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS02210	480193..480912	-	720	WP_000611288.1	two-component system response regulator DcuR	-
CR208_RS02215	480909..482540	-	1632	WP_032317150.1	sensor histidine kinase	-
CR208_RS02220	482721..482951	+	231	WP_000371700.1	(4Fe-4S)-binding protein	-
CR208_RS02225	482963..483235	+	273	WP_000405647.1	N-acetyltransferase	-
CR208_RS02230	483462..483758	+	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
CR208_RS02235	483786..483959	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
CR208_RS02240	484078..485595	-	1518	WP_001295087.1	lysine--tRNA ligase	-
CR208_RS02245	485832..487289	-	1458	WP_000856841.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020087 4238430..4247780 0.007 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021458 4357262..4366612 0.007 View NC_012759 Escherichia coli BW2952 Genomic island TA023874 4289342..4298692 0.007 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285032 4238430..4247780 0.007	inside	Genomic island	-	-	483462..492812	9350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T81582 WP_001173343.1 NZ_CP023541:483786-483959 [Escherichia coli O104:H21 str. CFSAN002236]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T81582 NZ_CP023541:483786-483959 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT81582 WP_000398619.1 NZ_CP023541:483462-483758 [Escherichia coli O104:H21 str. CFSAN002236]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT81582 NZ_CP023541:483462-483758 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41

Antitoxin

• AlphaFold DB: A0A7U9QBH6