Detailed information of TA system TA081581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 201080..201682
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CR208_RS00975
• Protein ID: WP_000897305.1
• Coordinates: 201080..201391 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CR208_RS00980
• Protein ID: WP_000356397.1
• Coordinates: 201392..201682 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS00950	196994..197593	+	600	WP_001295269.1	glucose-1-phosphatase	-
CR208_RS00955	197587..198459	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CR208_RS00960	198456..198893	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CR208_RS00965	198938..199879	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
CR208_RS00970	199943..200851	-	909	WP_021293166.1	alpha/beta hydrolase	-
CR208_RS00975	201080..201391	+	312	WP_000897305.1	hypothetical protein	Toxin
CR208_RS00980	201392..201682	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CR208_RS00985	202287..202505	+	219	WP_021293459.1	ribbon-helix-helix domain-containing protein	-
CR208_RS00990	202725..202967	+	243	WP_001087409.1	hypothetical protein	-
CR208_RS00995	203297..204226	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CR208_RS01000	204223..204858	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CR208_RS01005	204855..205757	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T81581 WP_000897305.1 NZ_CP023541:201080-201391 [Escherichia coli O104:H21 str. CFSAN002236]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T81581 NZ_CP023541:201080-201391 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT81581 WP_000356397.1 NZ_CP023541:201392-201682 [Escherichia coli O104:H21 str. CFSAN002236]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT81581 NZ_CP023541:201392-201682 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7