Detailed information of TA system TA081566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3330315..3330917
• Replicon: chromosome
• Accession: NZ_CP023535
• Organism: Escherichia coli strain FDAARGOS_403

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CO706_RS18940
• Protein ID: WP_000897305.1
• Coordinates: 3330315..3330626 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CO706_RS18945
• Protein ID: WP_000356397.1
• Coordinates: 3330627..3330917 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO706_RS18915	3326229..3326828	+	600	WP_001295269.1	glucose-1-phosphatase	-
CO706_RS18920	3326822..3327694	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CO706_RS18925	3327691..3328128	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CO706_RS18930	3328173..3329114	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CO706_RS18935	3329178..3330086	-	909	WP_001387201.1	alpha/beta hydrolase	-
CO706_RS18940	3330315..3330626	+	312	WP_000897305.1	hypothetical protein	Toxin
CO706_RS18945	3330627..3330917	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CO706_RS18955	3331503..3331721	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CO706_RS18960	3331941..3332183	+	243	WP_001087409.1	hypothetical protein	-
CO706_RS18965	3332513..3333442	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CO706_RS18970	3333439..3334074	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CO706_RS18975	3334071..3334973	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T81566 WP_000897305.1 NZ_CP023535:3330315-3330626 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T81566 NZ_CP023535:3330315-3330626 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT81566 WP_000356397.1 NZ_CP023535:3330627-3330917 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT81566 NZ_CP023535:3330627-3330917 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7