Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1159884..1160386 Replicon chromosome
Accession NZ_CP023535
Organism Escherichia coli strain FDAARGOS_403

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CO706_RS07785 Protein ID WP_000767829.1
Coordinates 1159884..1160138 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CO706_RS07790 Protein ID WP_001259255.1
Coordinates 1160135..1160386 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO706_RS07760 1154899..1155126 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CO706_RS07765 1155140..1156198 - 1059 WP_000492324.1 transport protein YeeE -
CO706_RS07770 1156377..1157735 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CO706_RS29280 1157725..1157787 - 63 WP_010723108.1 membrane protein YoeI -
CO706_RS07775 1158002..1158931 - 930 WP_000803366.1 LysR family transcriptional regulator -
CO706_RS07780 1158977..1159801 - 825 WP_000754758.1 SDR family oxidoreductase -
CO706_RS07785 1159884..1160138 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CO706_RS07790 1160135..1160386 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CO706_RS07795 1160670..1160720 + 51 WP_001364200.1 his operon leader peptide -
CO706_RS07805 1160866..1161765 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CO706_RS07810 1161771..1163075 + 1305 WP_001386882.1 histidinol dehydrogenase -
CO706_RS07815 1163072..1164142 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
CO706_RS07820 1164142..1165209 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T81559 WP_000767829.1 NZ_CP023535:c1160138-1159884 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T81559 NZ_CP023535:c1160138-1159884 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT81559 WP_001259255.1 NZ_CP023535:c1160386-1160135 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT81559 NZ_CP023535:c1160386-1160135 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References