Detailed information of TA system TA081546

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43035..43299
• Replicon: plasmid unnamed1
• Accession: NZ_CP023534
• Organism: Escherichia coli strain FDAARGOS_403

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CO706_RS00885
• Protein ID: WP_001387489.1
• Coordinates: 43035..43187 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43237..43299 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO706_RS00860	38527..40695	+	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-
CO706_RS00865	40771..41385	+	615	WP_000578648.1	plasmid IncI1-type surface exclusion protein ExcA	-
CO706_RS00870	41483..41692	+	210	WP_001140543.1	hemolysin expression modulator Hha	-
CO706_RS29680	41875..42051	+	177	WP_001054898.1	hypothetical protein	-
CO706_RS00875	42116..42412	-	297	WP_001275298.1	hypothetical protein	-
CO706_RS00880	42712..42963	+	252	WP_001291964.1	hypothetical protein	-
CO706_RS00885	43035..43187	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	43237..43299	+	63	NuclAT_0	-	Antitoxin
-	43237..43299	+	63	NuclAT_0	-	Antitoxin
-	43237..43299	+	63	NuclAT_0	-	Antitoxin
-	43237..43299	+	63	NuclAT_0	-	Antitoxin
CO706_RS00890	43502..44593	+	1092	WP_000426061.1	hypothetical protein	-
CO706_RS00895	44900..46108	+	1209	WP_000121263.1	IncI1-type conjugal transfer protein TrbA	-
CO706_RS00900	46127..47197	+	1071	WP_000151588.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-15	-	1..108679	108679

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T81546 WP_001387489.1 NZ_CP023534:c43187-43035 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T81546 NZ_CP023534:c43187-43035 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT81546 NZ_CP023534:43237-43299 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin