Detailed information of TA system TA081540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4124694..4125296
• Replicon: chromosome
• Accession: NZ_CP023531
• Organism: Escherichia coli strain FDAARGOS_401

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CO703_RS22220
• Protein ID: WP_000897305.1
• Coordinates: 4124985..4125296 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CO703_RS22215
• Protein ID: WP_000356397.1
• Coordinates: 4124694..4124984 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO703_RS22185	4120638..4121540	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CO703_RS22190	4121537..4122172	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CO703_RS22195	4122169..4123098	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CO703_RS22200	4123428..4123670	-	243	WP_001087409.1	hypothetical protein	-
CO703_RS22205	4123890..4124108	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CO703_RS22215	4124694..4124984	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CO703_RS22220	4124985..4125296	-	312	WP_000897305.1	hypothetical protein	Toxin
CO703_RS22225	4125525..4126433	+	909	WP_001387201.1	alpha/beta hydrolase	-
CO703_RS22230	4126497..4127438	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CO703_RS22235	4127483..4127920	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CO703_RS22240	4127917..4128789	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CO703_RS22245	4128783..4129382	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T81540 WP_000897305.1 NZ_CP023531:c4125296-4124985 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T81540 NZ_CP023531:c4125296-4124985 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT81540 WP_000356397.1 NZ_CP023531:c4124984-4124694 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT81540 NZ_CP023531:c4124984-4124694 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7