Detailed information of TA system TA081488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1678684..1679418
• Replicon: chromosome
• Accession: NZ_CP023527
• Organism: Citrobacter koseri strain FDAARGOS_393

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CO700_RS08080
• Protein ID: WP_058668984.1
• Coordinates: 1678684..1678989 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A8AI81
• Locus tag: CO700_RS08085
• Protein ID: WP_012132926.1
• Coordinates: 1679077..1679418 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO700_RS08055	1674020..1674739	+	720	WP_058668981.1	aspartate/glutamate racemase family protein	-
CO700_RS08060	1674768..1675775	+	1008	WP_049009338.1	ABC transporter substrate-binding protein	-
CO700_RS08065	1675772..1676542	+	771	WP_058668982.1	ABC transporter permease	-
CO700_RS08070	1676542..1677318	+	777	WP_012132930.1	ABC transporter ATP-binding protein	-
CO700_RS08075	1677352..1678455	-	1104	WP_058668983.1	4Fe-4S binding protein	-
CO700_RS08080	1678684..1678989	+	306	WP_058668984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO700_RS08085	1679077..1679418	+	342	WP_012132926.1	HigA family addiction module antidote protein	Antitoxin
CO700_RS08090	1679558..1679863	-	306	WP_047457663.1	chaperone modulator CbpM	-
CO700_RS08095	1679863..1680783	-	921	WP_049009332.1	curved DNA-binding protein	-
CO700_RS08100	1681013..1681378	+	366	WP_047457658.1	hypothetical protein	-
CO700_RS08105	1681427..1683313	+	1887	WP_060816117.1	thioredoxin family protein	-
CO700_RS08110	1683310..1683933	+	624	WP_060816116.1	thioredoxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11958.68 Da
• Isoelectric Point: 8.3565

>T81488 WP_058668984.1 NZ_CP023527:1678684-1678989 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKVPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Nucleotide

• Length: 306 bp

>T81488 NZ_CP023527:1678684-1678989 [Citrobacter koseri]
ATGCCAAAGAAACTTGTCAGTATTGAAGACTTCCGTGACCAGTGGCTGGATGATTTTTTTATGTACTCTGCACCACATAA
GAAAGTTCCCCCGGATATACATTCAGCATTGGCCCGTAAGCTCGATATCATAAACGCGGCAGTGAGCTACAAGGATTTGA
AGTCACCGCCGGGTAACAGATACGAAGAACTGGAAGGTAAATTGCAGGAATATTCTTCGATAAGAGTTAACAAGCAATAT
CGTTTGATATTCAAATGGGTGAACGGCAAGGCACAAGACCTCTACCTTGATCCACATGAGTATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13062.91 Da
• Isoelectric Point: 5.1864

>AT81488 WP_012132926.1 NZ_CP023527:1679077-1679418 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Nucleotide

• Length: 342 bp

>AT81488 NZ_CP023527:1679077-1679418 [Citrobacter koseri]
ATGAACCAGGCAACCAGAAAACCCACGACAGTTGGTGATATCCTTCTGTATGAATACCTGGAACCGCTTGACCTCAAAAT
TAATGATCTCGCGGAAATGTTGCAGGTGCATCGTAATACCGCCAGCGCATTGGTTAACAACAATCGTAAACTCACGACTG
ACATGGCTTTTCGCCTGTCTAAAGTTTTCGATACCTCTGTAGAGTTCTGGATGAACCTACAGACTGCTGTAGATGTATGG
GAAACTGAAAACAATGCCCGCCTTCAGGTAGAGATGAGCAGGGTGATTACGGCGAGCGATTTTCTTGCGGAACGTCAGAA
ACAAGAAAAGAAAGTCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A8AI81