Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 90364..91082 Replicon chromosome
Accession NZ_CP023525
Organism Cedecea neteri strain FDAARGOS_392

Toxin (Protein)


Gene name higB Uniprot ID A0A089Q574
Locus tag CO704_RS00450 Protein ID WP_038477862.1
Coordinates 90771..91082 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A291DSN8
Locus tag CO704_RS00445 Protein ID WP_061277681.1
Coordinates 90364..90774 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO704_RS00420 86815..87456 + 642 WP_061277685.1 FMN-binding negative transcriptional regulator -
CO704_RS00425 87463..88512 - 1050 WP_061277684.1 AI-2E family transporter -
CO704_RS00430 88642..89199 - 558 WP_061277683.1 DcrB family lipoprotein -
CO704_RS00435 89272..89937 - 666 WP_045784268.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -
CO704_RS00440 90112..90357 + 246 WP_061277682.1 sulfurtransferase TusA -
CO704_RS00445 90364..90774 - 411 WP_061277681.1 helix-turn-helix domain-containing protein Antitoxin
CO704_RS00450 90771..91082 - 312 WP_038477862.1 type II toxin-antitoxin system HigB family toxin Toxin
CO704_RS00455 91225..93438 - 2214 WP_061277680.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
CO704_RS00460 93623..94669 + 1047 WP_061277679.1 LacI family transcriptional regulator -
CO704_RS00465 94833..95903 + 1071 WP_061277678.1 SIS domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-91)

Antitoxin

Cro/C1-type helix-turn-helix domain

(81-135)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12406.59 Da        Isoelectric Point: 10.6835

>T81464 WP_038477862.1 NZ_CP023525:c91082-90771 [Cedecea neteri]
MHLITLKTLIEAGQRFPRHRQELQILGKVLEKGFFPTPESLKRIYPSLDNFKYLDKHYVINIAGNELRLIALIFFTSQKF
YIRHIMTHSEYMRFTETHRGKKR

Download         Length: 312 bp

>T81464 NZ_CP023525:c91082-90771 [Cedecea neteri]
ATGCATCTGATTACGCTGAAGACGCTTATAGAAGCGGGTCAAAGATTTCCCAGACACAGGCAGGAACTGCAAATTCTCGG
CAAAGTGCTGGAAAAAGGCTTCTTTCCAACGCCGGAATCACTTAAGCGGATTTATCCTTCACTGGATAACTTTAAGTACC
TCGATAAACATTACGTCATCAATATCGCCGGCAATGAACTCAGGCTGATTGCGCTTATCTTTTTTACCAGCCAGAAGTTC
TACATCCGCCATATCATGACGCACAGCGAATACATGCGGTTTACTGAAACACACCGGGGAAAGAAGAGATGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15220.62 Da        Isoelectric Point: 6.2387

>AT81464 WP_061277681.1 NZ_CP023525:c90774-90364 [Cedecea neteri]
MIADALKAAEALKTAVPLLGGSTREKDYLAALELVEYLLLNDPENPLVEIVSSKISAWENAQPEVQTFRAEMQAIPAGIV
MLKTLMEHHQLKQSDFQQEIGSKSMVSRILSGERQLTTEHIRRLSRRFGISPALFF

Download         Length: 411 bp

>AT81464 NZ_CP023525:c90774-90364 [Cedecea neteri]
ATGATTGCTGATGCGCTTAAAGCCGCCGAAGCATTGAAAACGGCGGTTCCACTGCTGGGTGGCAGCACCAGAGAAAAAGA
TTATCTGGCGGCGCTGGAGCTGGTCGAATACCTGCTGCTCAATGACCCAGAAAATCCACTGGTTGAGATTGTCAGCAGCA
AAATCTCTGCCTGGGAAAACGCACAGCCTGAAGTACAGACGTTCCGAGCGGAAATGCAGGCAATCCCGGCCGGGATAGTC
ATGCTGAAAACCCTGATGGAGCATCACCAATTAAAACAGAGCGATTTTCAGCAAGAGATTGGCAGCAAGTCGATGGTATC
CCGCATTCTCAGCGGCGAGCGACAGCTAACCACCGAACACATCCGCCGGCTTTCCCGGCGCTTTGGCATCTCACCTGCCC
TCTTTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A089Q574

Antitoxin

Source ID Structure
AlphaFold DB A0A291DSN8

References