Detailed information of TA system TA081458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 546523..547134
• Replicon: chromosome
• Accession: NZ_CP023523
• Organism: Burkholderia gladioli pv. gladioli strain FDAARGOS_389

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CO712_RS21545
• Protein ID: WP_096751068.1
• Coordinates: 546865..547134 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A560VTG3
• Locus tag: CO712_RS21540
• Protein ID: WP_029951048.1
• Coordinates: 546523..546846 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO712_RS21520	542237..542650	-	414	WP_013690200.1	MarR family transcriptional regulator	-
CO712_RS21525	542758..543936	-	1179	WP_096751067.1	HPP family protein	-
CO712_RS21530	544331..545287	+	957	WP_013690202.1	LysR family transcriptional regulator	-
CO712_RS21535	545330..546415	-	1086	WP_036056417.1	ionic transporter y4hA	-
CO712_RS21540	546523..546846	-	324	WP_029951048.1	HigA family addiction module antidote protein	Antitoxin
CO712_RS21545	546865..547134	-	270	WP_096751068.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO712_RS21550	547377..548045	+	669	WP_025100406.1	NAAT family transporter	-
CO712_RS21555	548166..549035	-	870	WP_096751069.1	CPBP family intramembrane metalloprotease	-
CO712_RS21560	549301..550023	-	723	WP_096751070.1	fumarylacetoacetate hydrolase family protein	-
CO712_RS21565	550182..551300	-	1119	WP_096751071.1	helix-turn-helix transcriptional regulator	-
CO712_RS21570	551493..552095	-	603	WP_096751072.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-87)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (13-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 9999.54 Da
• Isoelectric Point: 10.8461

>T81458 WP_096751068.1 NZ_CP023523:c547134-546865 [Burkholderia gladioli pv. gladioli]
MIQSFRCKRTAALFAGESPPAWRAVAQVARRKLAMLDAAQALRDLAVPPGNQLEGLFGDRAGQYSIRINERWRLCFVRSG
RGPELVDYH

Nucleotide

• Length: 270 bp

>T81458 NZ_CP023523:c547134-546865 [Burkholderia gladioli pv. gladioli]
ATGATCCAGTCATTCCGATGCAAGCGCACCGCCGCGCTGTTCGCCGGCGAATCGCCGCCGGCCTGGCGTGCCGTGGCACA
AGTGGCCCGGCGCAAGCTGGCCATGCTCGATGCGGCCCAGGCGCTGCGCGACCTGGCCGTGCCGCCCGGCAACCAGCTCG
AAGGCCTGTTCGGCGATCGCGCGGGGCAATACAGCATCCGGATCAACGAGCGCTGGCGCCTCTGTTTCGTCCGGAGCGGC
CGGGGGCCCGAGCTCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 7.2024

>AT81458 WP_029951048.1 NZ_CP023523:c546846-546523 [Burkholderia gladioli pv. gladioli]
MNAKSVKPVNAMPAIHPGEILREEFLVPLALSVNALSLALMVPATRMHEIVNERRGITADTAYRLSRYFGTTPEFWLALQ
ADYDIKTLPNRDEIDRKVDARPNGAAA

Nucleotide

• Length: 324 bp

>AT81458 NZ_CP023523:c546846-546523 [Burkholderia gladioli pv. gladioli]
ATGAATGCCAAGAGTGTGAAGCCCGTCAACGCGATGCCCGCCATCCACCCCGGCGAAATCCTGCGCGAGGAGTTCCTGGT
GCCGCTCGCGCTCAGCGTCAACGCGCTGTCGCTGGCGCTGATGGTGCCGGCCACCCGCATGCACGAGATCGTCAACGAGC
GGCGCGGCATCACCGCCGACACGGCCTACCGCCTGTCGCGCTACTTCGGCACCACGCCGGAGTTCTGGCTGGCCCTGCAG
GCCGACTACGACATCAAGACCCTGCCGAACCGCGACGAGATCGACCGCAAGGTCGATGCGCGCCCCAACGGCGCGGCGGC
CTGA

Structures

Antitoxin

• AlphaFold DB: A0A560VTG3