Detailed information of TA system TA081442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1211776..1212543
• Replicon: chromosome
• Accession: NZ_CP023516
• Organism: Pasteurella multocida strain FDAARGOS_384

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J9QLH7
• Locus tag: CO688_RS05730
• Protein ID: WP_005725755.1
• Coordinates: 1211776..1212102 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CO688_RS05735
• Protein ID: WP_096743450.1
• Coordinates: 1212217..1212543 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO688_RS05705	1207093..1207779	+	687	WP_061406323.1	hypothetical protein	-
CO688_RS05710	1207833..1209362	-	1530	WP_096743448.1	YifB family Mg chelatase-like AAA ATPase	-
CO688_RS05715	1209471..1210088	-	618	WP_005724253.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
CO688_RS05720	1210199..1211068	+	870	WP_096743449.1	VirK/YbjX family protein	-
CO688_RS05725	1211142..1211618	-	477	WP_042742721.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
CO688_RS05730	1211776..1212102	+	327	WP_005725755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO688_RS05735	1212217..1212543	+	327	WP_096743450.1	HigA family addiction module antidote protein	Antitoxin
CO688_RS05740	1212598..1214118	-	1521	WP_096743451.1	DUF560 domain-containing protein	-
CO688_RS05745	1214177..1214767	-	591	WP_096744022.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
CO688_RS11335	1214830..1215846	-	1017	WP_172459827.1	protein PlpE	-
CO688_RS05755	1216093..1217124	-	1032	WP_096743452.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12892.73 Da
• Isoelectric Point: 9.6705

>T81442 WP_005725755.1 NZ_CP023516:1211776-1212102 [Pasteurella multocida]
MDRVRFNLTEDSFQDKYLYEYFLYGSKHKNIPSDIENTLAHKLDMLDSANTLSDLRSPPGNKLEKLEPKTSGLYSIRVNV
KYRLIFKYKESNFPKITELRLDKHQYRL

Nucleotide

• Length: 327 bp

>T81442 NZ_CP023516:1211776-1212102 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGCG
ATTTGCGAAGTCCACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12387.45 Da
• Isoelectric Point: 7.2034

>AT81442 WP_096743450.1 NZ_CP023516:1212217-1212543 [Pasteurella multocida]
MSRVQRKPSTVGDILLDEYLVPLNLKIADLATMLDVHRNTASALVNNNTKLSLEMALKLAKLFNTSPEFWLNLQMKLDLW
EIENNARFQESLKKISPIDQHNLLEKIA

Nucleotide

• Length: 327 bp

>AT81442 NZ_CP023516:1212217-1212543 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCGAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGCCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2J9QLH7