Detailed information of TA system TA081432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4816115..4816781
• Replicon: chromosome
• Accession: NZ_CP023504
• Organism: Citrobacter werkmanii strain FDAARGOS_364

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CO701_RS23745
• Protein ID: WP_096759269.1
• Coordinates: 4816464..4816781 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: CO701_RS23740
• Protein ID: WP_003837894.1
• Coordinates: 4816115..4816411 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO701_RS23725	4813291..4813764	-	474	WP_003023524.1	transcription elongation factor GreB	-
CO701_RS23730	4813991..4814710	+	720	WP_001157751.1	two-component system response regulator OmpR	-
CO701_RS23735	4814707..4816059	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
CO701_RS23740	4816115..4816411	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
CO701_RS23745	4816464..4816781	-	318	WP_096759269.1	toxin HigB-2	Toxin
CO701_RS23750	4816905..4818527	-	1623	WP_096759270.1	phosphoenolpyruvate carboxykinase (ATP)	-
CO701_RS23755	4818906..4820624	+	1719	WP_096759271.1	DUF4153 domain-containing protein	-
CO701_RS23760	4820675..4821553	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12213.13 Da
• Isoelectric Point: 9.8310

>T81432 WP_096759269.1 NZ_CP023504:c4816781-4816464 [Citrobacter werkmanii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIETPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T81432 NZ_CP023504:c4816781-4816464 [Citrobacter werkmanii]
ATGTTCACATTTATTGAGCTACAGGGATTTAGTAAACGCCGTCCGTTACTTTTACCTGACGATGAGTTTCGTGCCTTTCA
AGAGGCGCTTATTGAAACCCCTGAGGCAGGGGATACTATCGCTGGTACGGGGGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGTAAGCGCAGCGGTATTCGGGTTATCTATTACAACGTGACGCGTAAAGGTCGAATCTATCTGGCTTTGCTC
TATCCTAAGAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCGATATGCTGATTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT81432 WP_003837894.1 NZ_CP023504:c4816411-4816115 [Citrobacter werkmanii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT81432 NZ_CP023504:c4816411-4816115 [Citrobacter werkmanii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTACGTATAGAGAAAGGTGAAGAAACACCTAA
ACCTGAACATGTACATACTTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCGGTTGGTGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAACCCACTGGAGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAACCCATTTATAATTAATCAATTAAAAGCAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5