Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4547757..4548354 Replicon chromosome
Accession NZ_CP023487
Organism Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AN663_RS23045 Protein ID WP_004142563.1
Coordinates 4548037..4548354 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AN663_RS23040 Protein ID WP_004142561.1
Coordinates 4547757..4548044 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN663_RS23010 4543838..4544086 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AN663_RS23015 4544104..4544445 - 342 WP_055314312.1 RamA family antibiotic efflux transcriptional regulator -
AN663_RS23020 4544476..4545591 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
AN663_RS23025 4545770..4546351 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
AN663_RS23030 4546351..4546719 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AN663_RS23035 4546839..4547492 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
AN663_RS23040 4547757..4548044 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AN663_RS23045 4548037..4548354 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN663_RS23050 4548539..4549582 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
AN663_RS23060 4550249..4551115 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
AN663_RS23065 4551224..4552651 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T81381 WP_004142563.1 NZ_CP023487:c4548354-4548037 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T81381 NZ_CP023487:c4548354-4548037 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT81381 WP_004142561.1 NZ_CP023487:c4548044-4547757 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT81381 NZ_CP023487:c4548044-4547757 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References