Detailed information of TA system TA081287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 890118..890698
• Replicon: chromosome
• Accession: NZ_CP023466
• Organism: Pseudomonas frederiksbergensis strain KNU-15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A291ABP7
• Locus tag: CNN82_RS04210
• Protein ID: WP_019581298.1
• Coordinates: 890420..890698 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNN82_RS04205
• Protein ID: WP_019581299.1
• Coordinates: 890118..890405 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNN82_RS04180	885396..886307	-	912	WP_028938866.1	LysR family transcriptional regulator	-
CNN82_RS04185	886440..887519	-	1080	WP_019581302.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
CNN82_RS04190	887516..888811	-	1296	WP_018925809.1	NCS2 family permease	-
CNN82_RS04195	889038..889493	+	456	WP_028938867.1	YolA family protein	-
CNN82_RS04200	889561..890112	+	552	WP_019581300.1	DJ-1/PfpI family protein	-
CNN82_RS04205	890118..890405	-	288	WP_019581299.1	HigA family addiction module antidote protein	Antitoxin
CNN82_RS04210	890420..890698	-	279	WP_019581298.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNN82_RS04215	890836..892080	-	1245	WP_019581297.1	MFS transporter	-
CNN82_RS04220	892460..893902	+	1443	WP_019581296.1	cytochrome ubiquinol oxidase subunit I	-
CNN82_RS04225	893906..894913	+	1008	WP_019581295.1	cytochrome d ubiquinol oxidase subunit II	-
CNN82_RS04230	894971..895138	+	168	WP_081625770.1	DUF2474 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10755.30 Da
• Isoelectric Point: 8.5218

>T81287 WP_019581298.1 NZ_CP023466:c890698-890420 [Pseudomonas frederiksbergensis]
MIKSFQHKGLRGFYETGSTRGIRADHAKRLARMLQFMDRAATPDDLDLPGWRLHPLKGDLVEYWSLSVSGNWRVIFRFIG
SDIELVDYLDYH

Nucleotide

• Length: 279 bp

>T81287 NZ_CP023466:c890698-890420 [Pseudomonas frederiksbergensis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTACGAAACCGGTTCGACTCGTGGGATTCGAGCAGATCATGC
GAAGCGTCTGGCTCGCATGCTGCAGTTTATGGATCGCGCAGCGACTCCCGACGACCTCGACCTTCCGGGGTGGCGATTAC
ATCCGCTCAAGGGCGATCTGGTCGAATATTGGTCACTCAGTGTTTCGGGGAACTGGCGGGTTATTTTTCGTTTTATTGGT
TCGGATATCGAACTGGTCGACTATCTGGACTATCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10604.28 Da
• Isoelectric Point: 6.6041

>AT81287 WP_019581299.1 NZ_CP023466:c890405-890118 [Pseudomonas frederiksbergensis]
MPMHNPPHPGETLLMDVLPELGISVTELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGVQTDYDLWQA
EQREQPLIEPIAVHA

Nucleotide

• Length: 288 bp

>AT81287 NZ_CP023466:c890405-890118 [Pseudomonas frederiksbergensis]
ATGCCCATGCACAACCCGCCACACCCTGGCGAAACACTTTTGATGGATGTATTGCCCGAGCTTGGCATCAGCGTCACCGA
ACTGGCCCGACACCTTGGATTCGCCCGCCCGCATCTTTCGCGAGTGCTGCACGGCCATGCGCCCATCAGTCCGGATCTGG
CGGTACGCCTGGAGCGGGCTGGAATCGGTAAGGCTCGTATGTGGTTAGGCGTTCAGACCGACTACGATCTCTGGCAGGCG
GAGCAGCGTGAGCAACCGCTCATCGAGCCGATTGCTGTCCACGCCTGA

Structures

Toxin

• AlphaFold DB: A0A291ABP7