Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 258323..258915 Replicon chromosome
Accession NZ_CP023466
Organism Pseudomonas frederiksbergensis strain KNU-15

Toxin (Protein)


Gene name higB Uniprot ID A0A291AA59
Locus tag CNN82_RS01155 Protein ID WP_026331819.1
Coordinates 258323..258601 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A291AAM5
Locus tag CNN82_RS01160 Protein ID WP_019580494.1
Coordinates 258610..258915 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNN82_RS01140 255051..256265 + 1215 WP_028939141.1 aldose 1-epimerase family protein -
CNN82_RS01145 256272..257765 - 1494 WP_096479376.1 YifB family Mg chelatase-like AAA ATPase -
CNN82_RS01150 257889..258074 - 186 WP_019580492.1 hypothetical protein -
CNN82_RS01155 258323..258601 + 279 WP_026331819.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CNN82_RS01160 258610..258915 + 306 WP_019580494.1 HigA family addiction module antidote protein Antitoxin
CNN82_RS01165 258968..259486 - 519 WP_096479377.1 Bro-N domain-containing protein -
CNN82_RS01170 259888..260154 - 267 WP_019580496.1 accessory factor UbiK family protein -
CNN82_RS01175 260571..260909 + 339 WP_002555808.1 P-II family nitrogen regulator -
CNN82_RS01180 260945..262282 + 1338 WP_007941275.1 ammonium transporter -
CNN82_RS01185 262496..262921 + 426 WP_018927529.1 secondary thiamine-phosphate synthase enzyme YjbQ -
CNN82_RS01190 263024..263347 + 324 WP_007941277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10550.22 Da        Isoelectric Point: 7.8601

>T81286 WP_026331819.1 NZ_CP023466:258323-258601 [Pseudomonas frederiksbergensis]
MIVGFKCSETEYLFRNGKTRMWSAILSVVERKLTMLDAAAVLMDLRSPPGNRLEALEGDRKGQHSIRINAQWRICFVWGL
NGPENVEIVDYH

Download         Length: 279 bp

>T81286 NZ_CP023466:258323-258601 [Pseudomonas frederiksbergensis]
ATGATCGTTGGCTTTAAGTGTTCTGAGACCGAGTACCTGTTTCGCAACGGAAAGACCCGTATGTGGTCAGCCATTCTGAG
TGTCGTGGAACGAAAGCTGACGATGCTTGATGCTGCTGCGGTGCTGATGGACCTTCGATCACCACCAGGCAATCGATTGG
AGGCGCTGGAAGGAGATCGAAAAGGTCAGCACAGCATCCGCATCAACGCTCAATGGCGAATTTGCTTTGTTTGGGGTCTT
AACGGACCAGAGAATGTCGAAATCGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11186.94 Da        Isoelectric Point: 7.1344

>AT81286 WP_019580494.1 NZ_CP023466:258610-258915 [Pseudomonas frederiksbergensis]
MTKNGMRPVHPGEILKEEYLEPLSLTAAALARALSVSTPTVNDIVLQRRGVSADMALRLSICLGTTPEFWLNLQSTYDLR
KAEIERGAAIRDQVERLPHCA

Download         Length: 306 bp

>AT81286 NZ_CP023466:258610-258915 [Pseudomonas frederiksbergensis]
ATGACTAAGAATGGTATGCGCCCGGTTCACCCTGGCGAGATCCTCAAAGAGGAATACCTGGAGCCTCTGAGCCTTACGGC
TGCAGCCTTGGCCAGAGCGTTGAGCGTTTCGACGCCTACTGTGAACGACATCGTACTACAGCGTCGAGGTGTCAGTGCCG
ATATGGCTCTAAGGCTATCTATCTGCCTGGGCACGACTCCCGAGTTTTGGCTGAACCTGCAATCGACATACGATTTGCGA
AAAGCCGAGATCGAACGAGGGGCTGCGATCCGGGATCAGGTTGAGCGGCTCCCGCACTGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291AA59

Antitoxin

Source ID Structure
AlphaFold DB A0A291AAM5

References