Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 182304..182839 Replicon plasmid pTT39_p1
Accession NZ_CP023424
Organism Enterococcus faecium strain K60-39

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag CNX66_RS14960 Protein ID WP_002314366.1
Coordinates 182304..182576 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag CNX66_RS14965 Protein ID WP_002297422.1
Coordinates 182576..182839 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNX66_RS14925 177861..178504 + 644 Protein_179 transposase -
CNX66_RS14930 178580..179260 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
CNX66_RS14935 179315..179671 - 357 Protein_181 hypothetical protein -
CNX66_RS14940 179931..180478 - 548 Protein_182 hypothetical protein -
CNX66_RS14945 180479..181336 - 858 WP_002292680.1 AAA family ATPase -
CNX66_RS14950 181622..181908 - 287 Protein_184 type II toxin-antitoxin system RelE/ParE family toxin -
CNX66_RS14955 181898..182227 - 330 WP_002292678.1 AbrB family transcriptional regulator -
CNX66_RS14960 182304..182576 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
CNX66_RS14965 182576..182839 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CNX66_RS14970 183073..183981 + 909 Protein_188 IS982 family transposase -
CNX66_RS14975 183984..185159 - 1176 WP_002336205.1 MFS transporter -
CNX66_RS14980 185162..187411 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / aph(2'')-Ia - 1..195692 195692
- inside IScluster/Tn - - 168881..193440 24559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T81251 WP_002314366.1 NZ_CP023424:c182576-182304 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T81251 NZ_CP023424:c182576-182304 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT81251 WP_002297422.1 NZ_CP023424:c182839-182576 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT81251 NZ_CP023424:c182839-182576 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References