Detailed information of TA system TA081245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 125527..126254
• Replicon: plasmid pKp1050-1
• Accession: NZ_CP023417
• Organism: Klebsiella pneumoniae strain 1050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: CN260_RS27580
• Protein ID: WP_011251285.1
• Coordinates: 125527..125838 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CN260_RS27585
• Protein ID: WP_011251286.1
• Coordinates: 125835..126254 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CN260_RS27550	121098..121945	+	848	Protein_122	DDE-type integrase/transposase/recombinase	-
CN260_RS27555	121965..122933	+	969	WP_167876016.1	IS5 family transposase	-
CN260_RS27560	122991..124040	-	1050	Protein_124	IS66 family transposase	-
CN260_RS27565	124089..124436	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
CN260_RS27570	124433..124771	-	339	WP_135733211.1	transposase	-
CN260_RS27575	124864..125322	+	459	Protein_127	DDE-type integrase/transposase/recombinase	-
CN260_RS27580	125527..125838	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
CN260_RS27585	125835..126254	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
CN260_RS27595	126401..127367	+	967	Protein_130	IS5 family transposase	-
CN260_RS27600	127401..127595	+	195	WP_110570734.1	DUF2188 domain-containing protein	-
CN260_RS27605	127695..128207	+	513	Protein_132	DUF4113 domain-containing protein	-
CN260_RS27610	128308..128751	+	444	WP_004181895.1	DUF2384 domain-containing protein	-
CN260_RS27615	128748..129218	+	471	WP_004197882.1	RES family NAD+ phosphorylase	-
CN260_RS27620	129329..129589	-	261	WP_004026352.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..311641	311641
	-	inside	IScluster/Tn	-	-	119749..131332	11583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T81245 WP_011251285.1 NZ_CP023417:125527-125838 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T81245 NZ_CP023417:125527-125838 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT81245 WP_011251286.1 NZ_CP023417:125835-126254 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT81245 NZ_CP023417:125835-126254 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1