Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2591865..2592388 Replicon chromosome
Accession NZ_CP023390
Organism Staphylococcus aureus subsp. aureus str. Newman strain NYU_Newman

Toxin (Protein)


Gene name yoeB Uniprot ID X5DYX7
Locus tag CNH35_RS13860 Protein ID WP_000074048.1
Coordinates 2591865..2592131 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag CNH35_RS13865 Protein ID WP_000587616.1
Coordinates 2592131..2592388 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNH35_RS13840 2587172..2588359 - 1188 WP_000026194.1 MFS transporter -
CNH35_RS13845 2588755..2589531 - 777 WP_000072147.1 iron export ABC transporter permease subunit FetB -
CNH35_RS13850 2589524..2590186 - 663 WP_000923520.1 ATP-binding cassette domain-containing protein -
CNH35_RS13855 2590434..2591510 - 1077 WP_001076671.1 M42 family metallopeptidase -
CNH35_RS13860 2591865..2592131 - 267 WP_000074048.1 Txe/YoeB family addiction module toxin Toxin
CNH35_RS13865 2592131..2592388 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CNH35_RS13870 2592494..2592688 - 195 WP_001790145.1 hypothetical protein -
CNH35_RS13875 2592747..2593202 + 456 WP_000732082.1 DUF1307 domain-containing protein -
CNH35_RS13880 2593370..2594947 - 1578 WP_000143503.1 FMN-binding glutamate synthase family protein -
CNH35_RS13885 2595142..2595891 + 750 WP_000072443.1 hypothetical protein -
CNH35_RS13890 2596115..2597308 - 1194 WP_000675401.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(25-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10648.23 Da        Isoelectric Point: 10.1384

>T81182 WP_000074048.1 NZ_CP023390:c2592131-2591865 [Staphylococcus aureus subsp. aureus str. Newman]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T81182 NZ_CP023390:c2592131-2591865 [Staphylococcus aureus subsp. aureus str. Newman]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT81182 WP_000587616.1 NZ_CP023390:c2592388-2592131 [Staphylococcus aureus subsp. aureus str. Newman]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT81182 NZ_CP023390:c2592388-2592131 [Staphylococcus aureus subsp. aureus str. Newman]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7V6W
PDB 7V5Y
PDB 7V5Z

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References