Detailed information of TA system TA081179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2535013..2535530
• Replicon: chromosome
• Accession: NZ_CP023390
• Organism: Staphylococcus aureus subsp. aureus str. Newman strain NYU_Newman

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: CNH35_RS13540
• Protein ID: WP_000113262.1
• Coordinates: 2535013..2535279 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: CNH35_RS13545
• Protein ID: WP_000584499.1
• Coordinates: 2535279..2535530 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNH35_RS13510	2530440..2530976	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
CNH35_RS13515	2531209..2532033	-	825	WP_000572040.1	formate/nitrite transporter family protein	-
CNH35_RS13520	2532250..2532432	-	183	WP_000230294.1	hypothetical protein	-
CNH35_RS13525	2532516..2532983	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
CNH35_RS13530	2533123..2534718	-	1596	WP_000727761.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
CNH35_RS13535	2534907..2534978	-	72	Protein_2513	hypothetical protein	-
CNH35_RS13540	2535013..2535279	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
CNH35_RS13545	2535279..2535530	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CNH35_RS13550	2535695..2535796	-	102	WP_001791744.1	hypothetical protein	-
CNH35_RS13555	2535803..2536402	-	600	WP_000162813.1	DsbA family protein	-
CNH35_RS13560	2536421..2536783	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
CNH35_RS13565	2537038..2538288	+	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
CNH35_RS13570	2538385..2539116	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
CNH35_RS13575	2539113..2539832	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T81179 WP_000113262.1 NZ_CP023390:c2535279-2535013 [Staphylococcus aureus subsp. aureus str. Newman]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T81179 NZ_CP023390:c2535279-2535013 [Staphylococcus aureus subsp. aureus str. Newman]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT81179 WP_000584499.1 NZ_CP023390:c2535530-2535279 [Staphylococcus aureus subsp. aureus str. Newman]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT81179 NZ_CP023390:c2535530-2535279 [Staphylococcus aureus subsp. aureus str. Newman]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60