Detailed information of TA system TA081161

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31721..31991
• Replicon: plasmid p74
• Accession: NZ_CP023389
• Organism: Escherichia coli strain 1105

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CNQ47_RS26135
• Protein ID: WP_001312861.1
• Coordinates: 31833..31991 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31721..31784 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ47_RS26910	27257..27463	+	207	WP_000275858.1	hypothetical protein	-
CNQ47_RS26110	27489..28028	+	540	WP_000290838.1	single-stranded DNA-binding protein	-
CNQ47_RS26115	28091..28324	+	234	WP_000005990.1	DUF905 family protein	-
CNQ47_RS26120	28390..30348	+	1959	WP_096058005.1	ParB/RepB/Spo0J family partition protein	-
CNQ47_RS26125	30403..30837	+	435	WP_096058006.1	conjugation system SOS inhibitor PsiB	-
CNQ47_RS26130	30834..31553	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CNQ47_RS26760	31565..31753	-	189	WP_001336239.1	hypothetical protein	-
-	31565..31789	+	225	NuclAT_0	-	-
-	31565..31789	+	225	NuclAT_0	-	-
-	31565..31789	+	225	NuclAT_0	-	-
-	31565..31789	+	225	NuclAT_0	-	-
-	31721..31784	-	64	-	-	Antitoxin
CNQ47_RS26135	31833..31991	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CNQ47_RS26140	32304..33875	-	1572	WP_096058007.1	IS66 family transposase	-
CNQ47_RS26145	33895..34242	-	348	WP_021557532.1	IS66 family insertion sequence element accessory protein TnpB	-
CNQ47_RS26150	34242..34892	-	651	WP_096058008.1	IS66-like element accessory protein TnpA	-
CNQ47_RS26155	34965..35153	+	189	WP_096058009.1	hypothetical protein	-
CNQ47_RS26170	35625..35912	+	288	WP_000107535.1	hypothetical protein	-
CNQ47_RS26175	36031..36852	+	822	WP_001234469.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..74094	74094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T81161 WP_001312861.1 NZ_CP023389:31833-31991 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T81161 NZ_CP023389:31833-31991 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT81161 NZ_CP023389:c31784-31721 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin