Detailed information of TA system TA081159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4886133..4886735
• Replicon: chromosome
• Accession: NZ_CP023388
• Organism: Escherichia coli strain 1105

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CNQ47_RS25035
• Protein ID: WP_000897302.1
• Coordinates: 4886424..4886735 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNQ47_RS25030
• Protein ID: WP_000356397.1
• Coordinates: 4886133..4886423 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ47_RS24995	4882207..4883109	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CNQ47_RS25000	4883106..4883741	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CNQ47_RS25005	4883738..4884667	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CNQ47_RS25010	4884883..4885101	-	219	WP_001683432.1	ribbon-helix-helix domain-containing protein	-
CNQ47_RS25020	4885497..4885774	-	278	Protein_4557	hypothetical protein	-
CNQ47_RS25030	4886133..4886423	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CNQ47_RS25035	4886424..4886735	-	312	WP_000897302.1	hypothetical protein	Toxin
CNQ47_RS25040	4886964..4887872	+	909	WP_001305059.1	alpha/beta hydrolase	-
CNQ47_RS25045	4887936..4888877	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CNQ47_RS25050	4888922..4889359	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CNQ47_RS25055	4889356..4890228	-	873	WP_001683429.1	virulence factor BrkB family protein	-
CNQ47_RS25060	4890222..4890821	-	600	WP_001314324.1	glucose-1-phosphatase	-
CNQ47_RS25065	4890920..4891705	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T81159 WP_000897302.1 NZ_CP023388:c4886735-4886424 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T81159 NZ_CP023388:c4886735-4886424 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT81159 WP_000356397.1 NZ_CP023388:c4886423-4886133 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT81159 NZ_CP023388:c4886423-4886133 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416