Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3899722..3900416 Replicon chromosome
Accession NZ_CP023388
Organism Escherichia coli strain 1105

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag CNQ47_RS20210 Protein ID WP_001263501.1
Coordinates 3899722..3900120 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CNQ47_RS20215 Protein ID WP_000554755.1
Coordinates 3900123..3900416 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ47_RS20180 3894960..3895418 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
CNQ47_RS20185 3895679..3897136 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
CNQ47_RS20190 3897193..3897807 - 615 WP_001682610.1 peptide chain release factor H -
CNQ47_RS20195 3897804..3898943 - 1140 WP_024194062.1 RNA ligase RtcB family protein -
CNQ47_RS20205 3899260..3899712 - 453 WP_001059867.1 GNAT family N-acetyltransferase -
CNQ47_RS20210 3899722..3900120 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CNQ47_RS20215 3900123..3900416 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CNQ47_RS20220 3900468..3901523 - 1056 WP_001226167.1 DNA polymerase IV -
CNQ47_RS20225 3901594..3902379 - 786 WP_000207543.1 putative lateral flagellar export/assembly protein LafU -
CNQ47_RS20230 3902351..3904063 + 1713 Protein_3686 flagellar biosynthesis protein FlhA -
CNQ47_RS26720 3904142..3904486 + 345 WP_143370186.1 hypothetical protein -
CNQ47_RS20235 3904481..3904978 - 498 WP_001682596.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T81151 WP_001263501.1 NZ_CP023388:c3900120-3899722 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T81151 NZ_CP023388:c3900120-3899722 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT81151 WP_000554755.1 NZ_CP023388:c3900416-3900123 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT81151 NZ_CP023388:c3900416-3900123 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGGTATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References