Detailed information of TA system TA081149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3676918..3677597
• Replicon: chromosome
• Accession: NZ_CP023388
• Organism: Escherichia coli strain 1105

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: CNQ47_RS19085
• Protein ID: WP_000057523.1
• Coordinates: 3677295..3677597 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: CNQ47_RS19080
• Protein ID: WP_000806442.1
• Coordinates: 3676918..3677259 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ47_RS19070	3673162..3674094	-	933	WP_000883041.1	glutaminase A	-
CNQ47_RS19075	3674356..3676860	+	2505	WP_000083983.1	copper-exporting P-type ATPase CopA	-
CNQ47_RS19080	3676918..3677259	-	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
CNQ47_RS19085	3677295..3677597	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ47_RS19090	3677730..3678524	+	795	WP_001682658.1	TraB/GumN family protein	-
CNQ47_RS19100	3678728..3679207	+	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
CNQ47_RS19105	3679244..3680896	-	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
CNQ47_RS19115	3681114..3682334	+	1221	WP_001251633.1	fosmidomycin MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T81149 WP_000057523.1 NZ_CP023388:c3677597-3677295 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T81149 NZ_CP023388:c3677597-3677295 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT81149 WP_000806442.1 NZ_CP023388:c3677259-3676918 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT81149 NZ_CP023388:c3677259-3676918 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3