81137

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2072609..2073111 Replicon chromosome
Accession NZ_CP023388
Organism Escherichia coli strain 1105

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CNQ47_RS10385 Protein ID WP_000767829.1
Coordinates 2072857..2073111 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CNQ47_RS10380 Protein ID WP_001259255.1
Coordinates 2072609..2072860 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ47_RS10350 2067787..2068854 - 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CNQ47_RS10355 2068854..2069924 - 1071 WP_001682999.1 histidinol-phosphate transaminase -
CNQ47_RS10360 2069921..2071225 - 1305 WP_001682998.1 histidinol dehydrogenase -
CNQ47_RS10365 2071231..2072130 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CNQ47_RS10375 2072276..2072326 - 51 WP_001364200.1 his operon leader peptide -
CNQ47_RS10380 2072609..2072860 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ47_RS10385 2072857..2073111 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ47_RS10390 2073194..2074018 + 825 WP_000754749.1 SDR family oxidoreductase -
CNQ47_RS10395 2074064..2074993 + 930 WP_000803351.1 LysR family transcriptional regulator -
CNQ47_RS26550 2075208..2075270 + 63 WP_010723108.1 membrane protein YoeI -
CNQ47_RS10400 2075260..2076618 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CNQ47_RS10405 2076797..2077855 + 1059 WP_000492315.1 transport protein YeeE -
CNQ47_RS10410 2077869..2078096 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T81137 WP_000767829.1 NZ_CP023388:2072857-2073111 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T81137 NZ_CP023388:2072857-2073111 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT81137 WP_001259255.1 NZ_CP023388:2072609-2072860 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT81137 NZ_CP023388:2072609-2072860 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References