Detailed information of TA system TA081121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3831009..3831659
• Replicon: chromosome
• Accession: NZ_CP023386
• Organism: Escherichia coli strain 1190

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CNQ48_RS19575
• Protein ID: WP_000263533.1
• Coordinates: 3831318..3831659 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D3GSB3
• Locus tag: CNQ48_RS19570
• Protein ID: WP_000212551.1
• Coordinates: 3831009..3831308 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ48_RS19540	3826457..3826768	-	312	WP_000414997.1	hypothetical protein	-
CNQ48_RS19545	3826773..3827165	-	393	WP_000285319.1	flagellar export chaperone FliS	-
CNQ48_RS19550	3827188..3828504	-	1317	WP_000609680.1	flagellar filament capping protein FliD	-
CNQ48_RS19560	3828709..3829623	-	915	WP_000949083.1	lateral flagellin LafA	-
CNQ48_RS19565	3830109..3830951	+	843	WP_122991190.1	winged helix-turn-helix domain-containing protein	-
CNQ48_RS19570	3831009..3831308	-	300	WP_000212551.1	XRE family transcriptional regulator	Antitoxin
CNQ48_RS19575	3831318..3831659	-	342	WP_000263533.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ48_RS19580	3831735..3832712	-	978	WP_001195947.1	hypothetical protein	-
CNQ48_RS19585	3832729..3833658	-	930	WP_001266806.1	flagellar hook-associated protein FlgL	-
CNQ48_RS19590	3833673..3835049	-	1377	WP_000367236.1	flagellar hook-associated protein FlgK	-
CNQ48_RS19595	3835255..3835554	-	300	WP_000867282.1	rod-binding protein	-
CNQ48_RS19600	3835554..3836654	-	1101	WP_001181883.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13047.86 Da
• Isoelectric Point: 6.4677

>T81121 WP_000263533.1 NZ_CP023386:c3831659-3831318 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLASMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T81121 NZ_CP023386:c3831659-3831318 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TTCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10976.81 Da
• Isoelectric Point: 9.7665

>AT81121 WP_000212551.1 NZ_CP023386:c3831308-3831009 [Escherichia coli]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT81121 NZ_CP023386:c3831308-3831009 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGCGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: D3GSB3