Detailed information of TA system TA081120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3820514..3821208
• Replicon: chromosome
• Accession: NZ_CP023386
• Organism: Escherichia coli strain 1190

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A1X0YXS1
• Locus tag: CNQ48_RS19500
• Protein ID: WP_001263484.1
• Coordinates: 3820514..3820912 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: CNQ48_RS19505
• Protein ID: WP_000554755.1
• Coordinates: 3820915..3821208 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ48_RS19470	3815684..3816928	-	1245	WP_000189541.1	esterase FrsA	-
-	3816344..3816424	-	81	NuclAT_8	-	-
-	3816344..3816424	-	81	NuclAT_8	-	-
-	3816344..3816424	-	81	NuclAT_8	-	-
-	3816344..3816424	-	81	NuclAT_8	-	-
CNQ48_RS19475	3817020..3817478	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CNQ48_RS19480	3817739..3819196	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CNQ48_RS19485	3819253..3819605	-	353	Protein_3591	peptide chain release factor H	-
CNQ48_RS19490	3819601..3819807	-	207	Protein_3592	RtcB family protein	-
CNQ48_RS19495	3820052..3820504	-	453	WP_001059899.1	GNAT family N-acetyltransferase	-
CNQ48_RS19500	3820514..3820912	-	399	WP_001263484.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CNQ48_RS19505	3820915..3821208	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CNQ48_RS19510	3821260..3822315	-	1056	WP_001226183.1	DNA polymerase IV	-
CNQ48_RS19515	3822386..3823309	-	924	WP_001232557.1	putative lateral flagellar export/assembly protein LafU	-
CNQ48_RS19520	3823312..3824175	-	864	WP_001169519.1	flagellar motor stator protein MotA	-
CNQ48_RS19525	3824188..3824904	-	717	WP_000938719.1	FliA/WhiG family RNA polymerase sigma factor	-
CNQ48_RS19530	3824924..3825391	-	468	WP_000725252.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15404.81 Da
• Isoelectric Point: 7.3840

>T81120 WP_001263484.1 NZ_CP023386:c3820912-3820514 [Escherichia coli]
MRVFKTKLICLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T81120 NZ_CP023386:c3820912-3820514 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTTGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT81120 WP_000554755.1 NZ_CP023386:c3821208-3820915 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT81120 NZ_CP023386:c3821208-3820915 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A1X0YXS1

Antitoxin

• AlphaFold DB: A0A829G9H5