81109

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1928889..1929391 Replicon chromosome
Accession NZ_CP023386
Organism Escherichia coli strain 1190

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CNQ48_RS09715 Protein ID WP_000767829.1
Coordinates 1929137..1929391 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A377LDC7
Locus tag CNQ48_RS09710 Protein ID WP_001259257.1
Coordinates 1928889..1929140 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ48_RS09680 1924067..1925134 - 1068 WP_000080080.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CNQ48_RS09685 1925134..1926204 - 1071 WP_000108953.1 histidinol-phosphate transaminase -
CNQ48_RS09690 1926201..1927505 - 1305 WP_001468917.1 histidinol dehydrogenase -
CNQ48_RS09695 1927511..1928410 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CNQ48_RS09705 1928556..1928606 - 51 WP_001364200.1 his operon leader peptide -
CNQ48_RS09710 1928889..1929140 + 252 WP_001259257.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ48_RS09715 1929137..1929391 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ48_RS09720 1929474..1930298 + 825 WP_000754737.1 SDR family oxidoreductase -
CNQ48_RS09725 1930344..1931273 + 930 WP_000803351.1 LysR family transcriptional regulator -
CNQ48_RS25665 1931488..1931550 + 63 WP_010723108.1 membrane protein YoeI -
CNQ48_RS09730 1931540..1932898 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T81109 WP_000767829.1 NZ_CP023386:1929137-1929391 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T81109 NZ_CP023386:1929137-1929391 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9306.64 Da        Isoelectric Point: 5.8289

>AT81109 WP_001259257.1 NZ_CP023386:1928889-1929140 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIK

Download         Length: 252 bp

>AT81109 NZ_CP023386:1928889-1929140 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A377LDC7

References