Detailed information of TA system TA081094

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108222..108647
• Replicon: plasmid p147
• Accession: NZ_CP023384
• Organism: Escherichia coli strain 1223

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: CNQ49_RS25030
• Protein ID: WP_001332444.1
• Coordinates: 108222..108383 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	108436..108647 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ49_RS25000	103572..103799	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
CNQ49_RS25005	103893..104579	-	687	WP_000332490.1	PAS domain-containing protein	-
CNQ49_RS25010	104769..105152	-	384	WP_070749934.1	relaxosome protein TraM	-
CNQ49_RS25015	105486..106076	+	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
CNQ49_RS25020	106372..107193	-	822	WP_001234485.1	DUF945 domain-containing protein	-
CNQ49_RS25025	107372..107984	+	613	Protein_108	hypothetical protein	-
CNQ49_RS25030	108222..108383	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	108436..108647	-	212	NuclAT_0	-	Antitoxin
-	108436..108647	-	212	NuclAT_0	-	Antitoxin
-	108436..108647	-	212	NuclAT_0	-	Antitoxin
-	108436..108647	-	212	NuclAT_0	-	Antitoxin
CNQ49_RS25040	108658..109377	-	720	WP_032163754.1	plasmid SOS inhibition protein A	-
CNQ49_RS25045	109374..109808	-	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
CNQ49_RS26295	109863..111821	-	1959	Protein_112	ParB/RepB/Spo0J family partition protein	-
CNQ49_RS25065	111880..112113	-	234	WP_001209608.1	DUF905 domain-containing protein	-
CNQ49_RS25070	112176..112673	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iucA	1..147369	147369
	-	flank	IS/Tn	-	-	113645..114022	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T81094 WP_001332444.1 NZ_CP023384:c108383-108222 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T81094 NZ_CP023384:c108383-108222 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT81094 NZ_CP023384:c108647-108436 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin