Detailed information of TA system TA081091

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71391..71645
• Replicon: plasmid p147
• Accession: NZ_CP023384
• Organism: Escherichia coli strain 1223

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CNQ49_RS24805
• Protein ID: WP_001351576.1
• Coordinates: 71391..71597 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	71584..71645 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ49_RS24765	68100..68305	-	206	Protein_62	hypothetical protein	-
CNQ49_RS24780	69219..70076	-	858	WP_096040816.1	incFII family plasmid replication initiator RepA	-
CNQ49_RS24785	70069..70551	-	483	WP_001273588.1	hypothetical protein	-
CNQ49_RS24790	70544..70618	-	75	WP_001442103.1	RepA leader peptide Tap	-
CNQ49_RS24800	70850..71107	-	258	WP_000083845.1	replication regulatory protein RepA	-
CNQ49_RS24805	71391..71597	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	71584..71645	+	62	NuclAT_1	-	Antitoxin
-	71584..71645	+	62	NuclAT_1	-	Antitoxin
-	71584..71645	+	62	NuclAT_1	-	Antitoxin
-	71584..71645	+	62	NuclAT_1	-	Antitoxin
CNQ49_RS26485	71901..71975	-	75	Protein_68	endonuclease	-
CNQ49_RS24820	72221..72433	-	213	WP_001299730.1	ANR family transcriptional regulator	-
CNQ49_RS24825	72569..73129	-	561	WP_000139344.1	fertility inhibition protein FinO	-
CNQ49_RS24830	73184..73930	-	747	WP_023352099.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iucA	1..147369	147369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T81091 WP_001351576.1 NZ_CP023384:c71597-71391 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T81091 NZ_CP023384:c71597-71391 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT81091 NZ_CP023384:71584-71645 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin