Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2805932..2806434 Replicon chromosome
Accession NZ_CP023383
Organism Escherichia coli strain 1223

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag CNQ49_RS14595 Protein ID WP_000767831.1
Coordinates 2805932..2806186 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CNQ49_RS14600 Protein ID WP_001259255.1
Coordinates 2806183..2806434 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ49_RS14570 2800947..2801174 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CNQ49_RS14575 2801188..2802246 - 1059 WP_032258476.1 transport protein YeeE -
CNQ49_RS14580 2802425..2803783 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CNQ49_RS26160 2803773..2803835 - 63 WP_010723108.1 membrane protein YoeI -
CNQ49_RS14585 2804050..2804979 - 930 WP_032258477.1 LysR family transcriptional regulator -
CNQ49_RS14590 2805025..2805849 - 825 WP_000754737.1 SDR family oxidoreductase -
CNQ49_RS14595 2805932..2806186 - 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ49_RS14600 2806183..2806434 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ49_RS14605 2806718..2806768 + 51 WP_001364200.1 his operon leader peptide -
CNQ49_RS14615 2806914..2807813 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CNQ49_RS14620 2807819..2809123 + 1305 WP_001565639.1 histidinol dehydrogenase -
CNQ49_RS14625 2809120..2810190 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
CNQ49_RS14630 2810190..2811257 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T81082 WP_000767831.1 NZ_CP023383:c2806186-2805932 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T81082 NZ_CP023383:c2806186-2805932 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT81082 WP_001259255.1 NZ_CP023383:c2806434-2806183 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT81082 NZ_CP023383:c2806434-2806183 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References