Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 989292..989986 Replicon chromosome
Accession NZ_CP023383
Organism Escherichia coli strain 1223

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CNQ49_RS04960 Protein ID WP_001263489.1
Coordinates 989588..989986 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CNQ49_RS04955 Protein ID WP_000554758.1
Coordinates 989292..989585 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ49_RS04935 984924..985421 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CNQ49_RS04940 985645..987357 - 1713 Protein_881 flagellar biosynthesis protein FlhA -
CNQ49_RS04945 987329..988114 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CNQ49_RS04950 988185..989240 + 1056 WP_001226164.1 DNA polymerase IV -
CNQ49_RS04955 989292..989585 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CNQ49_RS04960 989588..989986 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CNQ49_RS04965 989996..990448 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
CNQ49_RS04970 990766..990972 + 207 Protein_887 RtcB family protein -
CNQ49_RS04975 990968..991489 + 522 Protein_888 peptide chain release factor H -
CNQ49_RS04980 991546..993003 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CNQ49_RS04985 993264..993722 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 994318..994398 + 81 NuclAT_11 - -
- 994318..994398 + 81 NuclAT_11 - -
- 994318..994398 + 81 NuclAT_11 - -
- 994318..994398 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T81068 WP_001263489.1 NZ_CP023383:989588-989986 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T81068 NZ_CP023383:989588-989986 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT81068 WP_000554758.1 NZ_CP023383:989292-989585 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT81068 NZ_CP023383:989292-989585 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References