Detailed information of TA system TA081054

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84599..85020
• Replicon: plasmid p123
• Accession: NZ_CP023378
• Organism: Escherichia coli strain 127

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CNQ50_RS25605
• Protein ID: WP_001302184.1
• Coordinates: 84862..85020 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	84599..84797 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ50_RS25565	80295..81689	+	1395	WP_001037799.1	carbohydrate porin	-
CNQ50_RS25570	81935..82039	-	105	Protein_91	thiosulfate reductase cytochrome B subunit	-
CNQ50_RS25580	82096..82789	+	694	Protein_92	IS1 family transposase	-
CNQ50_RS25585	82805..83428	+	624	WP_000912556.1	potassium channel family protein	-
CNQ50_RS25590	83513..83764	+	252	Protein_94	iron-containing alcohol dehydrogenase	-
CNQ50_RS25595	84053..84370	-	318	Protein_95	IS110 family transposase	-
CNQ50_RS25600	84525..84630	+	106	Protein_96	plasmid SOS inhibition protein A	-
-	84599..84797	+	199	NuclAT_0	-	Antitoxin
-	84599..84797	+	199	NuclAT_0	-	Antitoxin
-	84599..84797	+	199	NuclAT_0	-	Antitoxin
-	84599..84797	+	199	NuclAT_0	-	Antitoxin
CNQ50_RS25605	84862..85020	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CNQ50_RS28215	85688..85894	+	207	WP_000275854.1	hypothetical protein	-
CNQ50_RS25620	85919..86206	+	288	WP_000107242.1	hypothetical protein	-
CNQ50_RS25625	86325..87146	+	822	WP_001234465.1	DUF945 domain-containing protein	-
CNQ50_RS25630	87442..88014	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CNQ50_RS25635	88384..88767	+	384	WP_001063020.1	relaxosome protein TraM	-
CNQ50_RS25640	88958..89605	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CNQ50_RS25645	89741..89956	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / dfrA17 / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / catA1	-	1..123289	123289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T81054 WP_001302184.1 NZ_CP023378:84862-85020 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T81054 NZ_CP023378:84862-85020 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT81054 NZ_CP023378:84599-84797 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin