Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4281147..4281644 Replicon chromosome
Accession NZ_CP023377
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CNQ50_RS22910 Protein ID WP_001173343.1
Coordinates 4281147..4281320 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CNQ50_RS22915 Protein ID WP_000398619.1
Coordinates 4281348..4281644 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS22895 4276498..4277955 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CNQ50_RS22900 4278192..4279709 + 1518 WP_001295074.1 lysine--tRNA ligase -
CNQ50_RS22905 4279881..4281109 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
CNQ50_RS22910 4281147..4281320 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CNQ50_RS22915 4281348..4281644 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CNQ50_RS22925 4281871..4282143 - 273 WP_000405647.1 N-acetyltransferase -
CNQ50_RS22930 4282155..4282385 - 231 WP_024250366.1 (4Fe-4S)-binding protein -
CNQ50_RS22935 4282566..4284197 + 1632 WP_001216477.1 sensor histidine kinase -
CNQ50_RS22940 4284194..4284913 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4272358..4281644 9286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T81051 WP_001173343.1 NZ_CP023377:c4281320-4281147 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T81051 NZ_CP023377:c4281320-4281147 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT81051 WP_000398619.1 NZ_CP023377:c4281644-4281348 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT81051 NZ_CP023377:c4281644-4281348 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References