Detailed information of TA system TA081047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3779172..3779866
• Replicon: chromosome
• Accession: NZ_CP023377
• Organism: Escherichia coli strain 127

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: CNQ50_RS20365
• Protein ID: WP_089578406.1
• Coordinates: 3779172..3779570 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CNQ50_RS20370
• Protein ID: WP_000554757.1
• Coordinates: 3779573..3779866 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ50_RS20335	3774172..3775416	-	1245	WP_000189541.1	esterase FrsA	-
-	3774832..3774912	-	81	NuclAT_12	-	-
-	3774832..3774912	-	81	NuclAT_12	-	-
-	3774832..3774912	-	81	NuclAT_12	-	-
-	3774832..3774912	-	81	NuclAT_12	-	-
CNQ50_RS20340	3775508..3775966	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CNQ50_RS20345	3776227..3777684	+	1458	WP_096037973.1	cytosol nonspecific dipeptidase	-
CNQ50_RS20350	3777741..3778262	-	522	Protein_3710	peptide chain release factor H	-
CNQ50_RS20355	3778261..3778464	-	204	Protein_3711	RNA ligase RtcB family protein	-
CNQ50_RS20360	3778710..3779162	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CNQ50_RS20365	3779172..3779570	-	399	WP_089578406.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CNQ50_RS20370	3779573..3779866	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CNQ50_RS20375	3779918..3780973	-	1056	WP_001226164.1	DNA polymerase IV	-
CNQ50_RS20380	3781044..3781829	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
CNQ50_RS20385	3781801..3783513	+	1713	Protein_3717	flagellar biosynthesis protein FlhA	-
CNQ50_RS20390	3783729..3784226	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3725408..3810616	85208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15506.85 Da
• Isoelectric Point: 8.0949

>T81047 WP_089578406.1 NZ_CP023377:c3779570-3779172 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHFVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T81047 NZ_CP023377:c3779570-3779172 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTTGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT81047 WP_000554757.1 NZ_CP023377:c3779866-3779573 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT81047 NZ_CP023377:c3779866-3779573 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6