Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1822833..1823335 Replicon chromosome
Accession NZ_CP023377
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CNQ50_RS09350 Protein ID WP_000767829.1
Coordinates 1823081..1823335 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CNQ50_RS09345 Protein ID WP_001259255.1
Coordinates 1822833..1823084 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS09315 1818010..1819077 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CNQ50_RS09320 1819077..1820147 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
CNQ50_RS09325 1820144..1821448 - 1305 WP_001446927.1 histidinol dehydrogenase -
CNQ50_RS09330 1821454..1822353 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CNQ50_RS09340 1822499..1822549 - 51 WP_001364200.1 his operon leader peptide -
CNQ50_RS09345 1822833..1823084 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ50_RS09350 1823081..1823335 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ50_RS09355 1823418..1824242 + 825 WP_000754737.1 SDR family oxidoreductase -
CNQ50_RS09360 1824288..1825217 + 930 WP_000803366.1 LysR family transcriptional regulator -
CNQ50_RS27780 1825432..1825494 + 63 WP_010723108.1 membrane protein YoeI -
CNQ50_RS09365 1825484..1826842 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CNQ50_RS09370 1827021..1828079 + 1059 WP_000492330.1 transport protein YeeE -
CNQ50_RS09375 1828093..1828320 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T81033 WP_000767829.1 NZ_CP023377:1823081-1823335 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T81033 NZ_CP023377:1823081-1823335 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT81033 WP_001259255.1 NZ_CP023377:1822833-1823084 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT81033 NZ_CP023377:1822833-1823084 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References