Detailed information of TA system TA080990

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31629..31893
• Replicon: plasmid p96
• Accession: NZ_CP023370
• Organism: Escherichia coli strain 1428

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CNQ52_RS27165
• Protein ID: WP_001387489.1
• Coordinates: 31741..31893 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31629..31689 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ52_RS27140	26701..27909	-	1209	WP_011264049.1	IncI1-type conjugal transfer protein TrbA	-
-	28089..28146	-	58	NuclAT_1	-	-
-	28089..28146	-	58	NuclAT_1	-	-
-	28089..28146	-	58	NuclAT_1	-	-
-	28089..28146	-	58	NuclAT_1	-	-
CNQ52_RS27145	28216..28965	-	750	Protein_35	protein finQ	-
CNQ52_RS27150	29194..29511	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
CNQ52_RS27155	29508..30041	-	534	WP_001221666.1	lipocalin family protein	-
CNQ52_RS27160	30135..31280	-	1146	WP_000976514.1	class C beta-lactamase CMY-2	-
-	31629..31689	-	61	NuclAT_0	-	Antitoxin
-	31629..31689	-	61	NuclAT_0	-	Antitoxin
-	31629..31689	-	61	NuclAT_0	-	Antitoxin
-	31629..31689	-	61	NuclAT_0	-	Antitoxin
CNQ52_RS27165	31741..31893	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
CNQ52_RS27170	31965..32216	-	252	WP_001291964.1	hypothetical protein	-
CNQ52_RS27175	32516..32812	+	297	WP_011264046.1	hypothetical protein	-
CNQ52_RS28110	32877..33053	-	177	WP_001054897.1	hypothetical protein	-
CNQ52_RS27180	33445..33654	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CNQ52_RS27185	33726..34388	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
CNQ52_RS27190	34459..36627	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..96402	96402

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T80990 WP_001387489.1 NZ_CP023370:31741-31893 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T80990 NZ_CP023370:31741-31893 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT80990 NZ_CP023370:c31689-31629 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin