Detailed information of TA system TA080988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2300..2946
• Replicon: plasmid p48
• Accession: NZ_CP023368
• Organism: Escherichia coli strain 1428

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CNQ52_RS26055
• Protein ID: WP_000269912.1
• Coordinates: 2300..2647 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNQ52_RS26060
• Protein ID: WP_001259435.1
• Coordinates: 2647..2946 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ52_RS26040	1..837	+	837	WP_001139599.1	replication initiator protein RepA	-
CNQ52_RS26045	1481..1768	+	288	WP_000356589.1	hypothetical protein	-
CNQ52_RS26050	1792..2055	+	264	WP_000424604.1	hypothetical protein	-
CNQ52_RS26055	2300..2647	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ52_RS26060	2647..2946	+	300	WP_001259435.1	XRE family transcriptional regulator	Antitoxin
CNQ52_RS26065	3110..3490	+	381	WP_000061761.1	hypothetical protein	-
CNQ52_RS26070	3594..4019	-	426	WP_016240784.1	hypothetical protein	-
CNQ52_RS26075	4121..4393	+	273	WP_042038402.1	helix-turn-helix domain-containing protein	-
CNQ52_RS26080	5004..5192	+	189	WP_004105254.1	hypothetical protein	-
CNQ52_RS26085	5203..6345	-	1143	WP_042038399.1	ORF6N domain-containing protein	-
CNQ52_RS26090	6342..6533	-	192	WP_042038396.1	hypothetical protein	-
CNQ52_RS28050	6644..6802	-	159	WP_157918775.1	ash family protein	-
CNQ52_RS26095	7663..7911	-	249	WP_000730008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48774	48774

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T80988 WP_000269912.1 NZ_CP023368:2300-2647 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T80988 NZ_CP023368:2300-2647 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11046.87 Da
• Isoelectric Point: 5.2090

>AT80988 WP_001259435.1 NZ_CP023368:2647-2946 [Escherichia coli]
MRTLDEAIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT80988 NZ_CP023368:2647-2946 [Escherichia coli]
ATGAGAACATTAGATGAGGCGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA