Detailed information of TA system TA080976

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4200513..4200925
• Replicon: chromosome
• Accession: NZ_CP023366
• Organism: Escherichia coli strain 1428

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: CNQ52_RS21165
• Protein ID: WP_000132601.1
• Coordinates: 4200584..4200925 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4200513..4200589 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ52_RS21155	4197376..4198965	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
CNQ52_RS21160	4198962..4200356	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
-	4200513..4200589	-	77	NuclAT_8	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_8	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_8	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_8	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_9	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_9	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_9	-	Antitoxin
-	4200513..4200589	-	77	NuclAT_9	-	Antitoxin
CNQ52_RS21165	4200584..4200925	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
CNQ52_RS21170	4201087..4202466	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
CNQ52_RS21175	4202466..4203512	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T80976 WP_000132601.1 NZ_CP023366:4200584-4200925 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T80976 NZ_CP023366:4200584-4200925 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT80976 NZ_CP023366:c4200589-4200513 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin