Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3859588..3860282 Replicon chromosome
Accession NZ_CP023366
Organism Escherichia coli strain 1428

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CNQ52_RS19520 Protein ID WP_001263491.1
Coordinates 3859588..3859986 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CNQ52_RS19525 Protein ID WP_000554755.1
Coordinates 3859989..3860282 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ52_RS19490 3854850..3856307 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
CNQ52_RS19495 3856316..3856597 + 282 WP_022645226.1 hypothetical protein -
CNQ52_RS19500 3856614..3857123 - 510 WP_001361775.1 hydrolase -
CNQ52_RS19505 3857185..3857799 - 615 WP_022645225.1 peptide chain release factor H -
CNQ52_RS19510 3857796..3858935 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
CNQ52_RS19515 3859126..3859578 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
CNQ52_RS19520 3859588..3859986 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CNQ52_RS19525 3859989..3860282 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CNQ52_RS19530 3860334..3861389 - 1056 WP_022645222.1 DNA polymerase IV -
CNQ52_RS19535 3861460..3862245 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
CNQ52_RS19540 3862217..3863929 + 1713 Protein_3613 flagellar biosynthesis protein FlhA -
CNQ52_RS19545 3864027..3864800 - 774 WP_022645219.1 C40 family peptidase -
CNQ52_RS19550 3864986..3865246 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T80974 WP_001263491.1 NZ_CP023366:c3859986-3859588 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T80974 NZ_CP023366:c3859986-3859588 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT80974 WP_000554755.1 NZ_CP023366:c3860282-3859989 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT80974 NZ_CP023366:c3860282-3859989 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References