Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1918064..1918566 Replicon chromosome
Accession NZ_CP023366
Organism Escherichia coli strain 1428

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CNQ52_RS09410 Protein ID WP_000767829.1
Coordinates 1918312..1918566 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CNQ52_RS09405 Protein ID WP_001259255.1
Coordinates 1918064..1918315 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ52_RS09375 1913242..1914309 - 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CNQ52_RS09380 1914309..1915379 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
CNQ52_RS09385 1915376..1916680 - 1305 WP_001559366.1 histidinol dehydrogenase -
CNQ52_RS09390 1916686..1917585 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CNQ52_RS09400 1917731..1917781 - 51 WP_001364200.1 his operon leader peptide -
CNQ52_RS09405 1918064..1918315 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ52_RS09410 1918312..1918566 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ52_RS09415 1918649..1919473 + 825 WP_000754749.1 SDR family oxidoreductase -
CNQ52_RS09420 1919519..1920448 + 930 WP_000803351.1 LysR family transcriptional regulator -
CNQ52_RS27695 1920663..1920725 + 63 WP_010723108.1 membrane protein YoeI -
CNQ52_RS09425 1920715..1922073 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CNQ52_RS09430 1922252..1923310 + 1059 WP_023910197.1 transport protein YeeE -
CNQ52_RS09435 1923324..1923551 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T80959 WP_000767829.1 NZ_CP023366:1918312-1918566 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80959 NZ_CP023366:1918312-1918566 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80959 WP_001259255.1 NZ_CP023366:1918064-1918315 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80959 NZ_CP023366:1918064-1918315 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References